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KMID : 0922220110110020050
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Journal of the Korean Musculoskeletal Transplantation Society
2011 Volume.11 No. 2 p.50 ~ p.54
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Bacterial Contamination Route of Allografts from Living Donors in Operating Room
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Hwang Jae-Kwang
Kim Chung-Hwan
Choi Young-Joon
Ahn Hyung-Sun
Lee Gi-Won
Kang Jeong-Ho
Park Jun-Seok
Choo Han-Ho
Choi Ji-Young
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Abstract
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Purpose: To identify the bacterial contamination at which point in the process of harvesting the bone tissue from living donor and wrapping the harvested bone tissue, and to find the methods for improvement of processing in relation with the contamination.
Materials and Methods: From October to December 2010, bone tissues were harvested at this hospital’s bone bank from 31 living donors who were adjudged to be eligible donor according to the criteria set in the bone bank’s standard operating procedure, and these bone tissues were used in this study. Bacterial culture test was performed on a total of 7 sites; surgical glove worn by surgeon, incision site, joint synovial fluid after the opening of the joint, immediate post-harvest bone tissue surface, normal saline for washing the harvested bone tissue, immediate pre-wrapping
bone tissue surface, and cotton swab before swabbing. Swabbing was performed using 2 swabs on each site, and a total of 14 specimens were prepared per case.
Results: In all cases, bacteria were not culture from cotton swab before swabbing, harvesting surgeon’s glove, incision site, joint synovial fluid, and immediate post-harvest bone tissue. Swab specimens from normal saline for washing bone tissue in 1 case(3.2%) and from immediate prewrapping bone tissue in 2 cases(6.4%) were positive for bacteria culture. Coagulase-nagative staphylococcus was identified in swab specimen obtained from immediate pre-wrapping bone tissue of 1 case, Gram positive coccci in immediate pre-wrapping bone tissue of 1 case, and
Enterococcus feacium in normal saline of 1 case.
Conclusion: These results suggest that bacterial contamination occurring during the process of harvesting allogenic bone from living donor could be attributable to final wrapping procedures after harvesting bone tissue.
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KEYWORD
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Bone banking, Bacterial contamination, Allogenic bone
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